Characterization and multiplexing of EST-SSR primers in Cynodon (Poaceae) species1
نویسندگان
چکیده
منابع مشابه
Development and characterization of EST-SSR markers in Stipa breviflora (Poaceae)1
PREMISE OF THE STUDY Stipa breviflora (Poaceae) is one of the dominant species of the desert steppe in the eastern Eurasian grasslands. Simple sequence repeat (SSR) markers were developed for use in genetic diversity studies of this species. METHODS AND RESULTS A total of 1954 potentially polymorphic loci were obtained by comparing transcriptome data of eight different S. breviflora individua...
متن کاملDevelopment and characterization of 29 polymorphic EST-SSR markers for Stipa purpurea (Poaceae)1
PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using Illumina sequencing for further genetic diversity studies of Stipa purpurea (Poaceae). METHODS AND RESULTS Twenty-nine polymorphic and eight monomorphic EST-SSR loci were developed and characterized in 90 individuals from nine S. purpurea populations. The number of alleles per locus range...
متن کاملDevelopment of EST-SSR markers in Barringtonia racemosa (Lecythidaceae) and cross-amplification in related species1
PREMISE OF THE STUDY Microsatellite markers were identified and characterized to study the genetic diversity and structure of Barringtonia racemosa (Lecythidaceae). METHODS AND RESULTS Based on the transcriptome data of B. racemosa, 30 primer pairs were initially designed and tested, of which 15 were successfully amplified and displayed clear polymorphisms across the 43 individuals from three...
متن کاملDevelopment of 32 EST-SSR markers for Abies firma (Pinaceae) and their transferability to related species1
UNLABELLED PREMISE OF THE STUDY We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Abies firma, a conifer endemic in Japan, to facilitate evaluation of the population genetic structure in this species. • METHODS AND RESULTS We designed primers for 153 EST-SSRs identified from 486322 ESTs from A. sachalinensis ESTs, and tested 96 of them for PCR amplif...
متن کاملKaryotype asymmetry in Cynodon Rich. (Poaceae) accessions.
Cynodon is a genus of plants with forage potential that has attracted the interest of breeders. These species have high morphological variability in a large number of varieties and cytotypes, hampering identification. This study aimed to determine the karyotype asymmetry index among accessions of Cynodon to discriminate between them. Karyotype symmetry was based on three estimates, which were c...
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ژورنال
عنوان ژورنال: American Journal of Botany
سال: 2010
ISSN: 0002-9122
DOI: 10.3732/ajb.1000254